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UHNMAC arrays: DNA methylation
home services platforms UHNMAC arrays: DNA methylation

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DMH enables researchers to identify changes in DNA methylation patterns, which is commonly observed in cancer and other disease states. For the DMH service, the researcher is responsible for preparing the genomic DNA, performing methylation-sensitive restrictions and PCR amplification. There are many protocols available in the literature. Currently, we follow the protocol outlined by Yan et al. Please refer to our scientific references page to learn more about differential methylation hybridisation.

Service includes:

• Amplified DNA quality checked with Nanodrop
• Labelling of two DNA samples per array with cyanine-3 and cyanine-5 (total input control/no-antibody control and one experimental). The UHNMAC may choose to substitute Alexa dyes for the cyanine dyes. For all experiments, the dyes will be kept consistent.
• Hybridisation/washing/scanning of arrays
• Image files sent to customer (CD or FTP)
• Spot quantification

We also offer an optional data analysis service. Please enquire directly for more information.

Please note: Due to reduced demand of our in-house printed cDNA microarrays, they will be discontinued by July 31st, 2010. They will only be made available for on-going projects with minimum order requirement. Please contact for details.

Pricing: Please contact us. (last updated: January 2011)
Please note that this pricing is for academic groups. Commercial customers should contact us for a quote.

Methylation experiments can be performed on Human CpG island arrays (15K, 12K, 8.1K) and Mouse CpG island arrays. Please see the microarray types page for more information on CpG island arrays.

Contact:

Please note that special paperwork is required for shipping biological samples from locations outside of Canada. For the Customs documentation that you will need, please download this form . Once you send us this information, the UHN Customs Officer will generate the appropriate Customs document and send it to you via e-mail. This document must be included with the waybill on your package.

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Methylation profiling is also available on the Agilent array platform.