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The UHNMAC prints a variety of arrays (human, mouse, yeast, custom) in-house. Our microarrays are printed on
UltraGAPS™ slides (Corning Inc.) using high-precision robotics. Printed arrays are processed following Corning's
protocol and are ready-to-use. To get pricing information, set up an account, or to place an order, please send
e-mail to
Please note: Due to reduced demand of our in-house printed cDNA microarrays, they have been
discontinued as of July 31st, 2010. They will only be made available for on-going projects with minimum order
requirement. Please contact
for details.
*Annotations on all arrays are subject to change. We recommend users update the latest annotations from the
corresponding databases. The gene lists are available here.
See our Ready-to-use Human Arrays Flyer
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Human Arrays
- 19K cDNA Array (H19K)
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- Single-spotted array containing 19,008 characterised and unknown human ESTs.*
- The clone set has been sequence-verified at the Microarray Centre and the sequences can be downloaded for validation.
- Can be hybridised "face-to-face" with the H8K array - see H27K Combo Array.
- 8.1K CpG-island Array (HCGI8.1K)
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- Single-spotted array containing 8,109 CpG-island clones consolidated from the UHN's HCGI12K set and an independent 8.5K set obtained from Tim Huang at the Ohio State University (Columbus, OH).*
- This library and array was described in Heisler L, et. al., CpG Island microarray probe sequences derived from a physical library are representative of CpG Islands annotated on the human genome. NAR (2005) 33:2952-2961
- Both sets were sequenced at the Michael Smith Genome Sciences Centre with support from the NIH/NCI, and here at the Microarray Centre. BLAT-annotated clones with overlapping sequences over 20% of either clone were considered as duplicates and excluded. Clones with internal repeat sequences, with no or more than one BLAT hits and those mapped to chromosome M (mitochondria) were also excluded. The resulting consolidated set contains 3,611 clones from the UHN's HCGI12K set and 4,498 from the 8.5K set. Visit our online database for clone annotations.
- Arabidopsis controls from the UHN Microarray Centre and clones from Stratagene's SpotReport Alien cDNA Array Validation System have been printed on the arrays.
- Since the HCGI8.1K arrays give consistently lower intensities, an indirect labeling protocol is recommended.
- 12K CpG-island Array (HCGI12K)
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- Single-spotted array containing 12,192 CpG-island clones from the Wellcome Trust Sanger Institute*
- The CpG Library was originally prepared by Sally Cross in the laboratory of Adrian Bird at The Wellcome Trust Centre for Cell Biology (University of Edinburgh). For information on the preparation of the original library, please see Cross, S.H., et.al., Purification of CpG islands using a methylated DNA binding column. Nature Genetics (1994) 6:236- 244.
- All clones were sequenced originally at the Wellcome Trust Sanger Institute and subsequently at the Michael Smith Genome Sciences Centre with support from the NIH/NCI and here at the Microarray Centre. Visit our online database for clone annotations.
- Arabadopsis controls from the UHN Microarray Centre have been added. In addition, Stratagene's SpotReport™ Alien™ cDNA Array Validation System has been printed onto the arrays.
Mouse Arrays
- 4.6K CpG-island Array (MCGI4.6K)
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- Single-spotted array containing 4,642 CpG-island clones obtained from the Sanger Institute.* This array set replaces MCGI7.3K.
- Based on sequencing and BLAT annotation, this set includes unique clones from the original MCGI7.3K and additional CpG-island clones.
- All clones were sequenced at the Michael Smith Genome Sciences Centre with support from NCI/NIH and here at the Microarray Centre. Visit our online database for clone annotations.
Other Arrays
- Yeast 6.4K Array (Y6.4K)
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- Double-spotted array containing 6,240 yeast ORFs (plus control spots totalling 6.4K).*
- Detailed information on the ORFs and sequences can be found at Saccharomyces Genome Database.
- Customised Arrays
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- We also provide custom printing services based on supplied material (cDNA or oligonucleotides). For details, please send e-mail to