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technical notes
The UHNMAC has evaluated new technologies, optimised microarray protocols and performed many in-house microarray experiments. In order to better support microarray users, we have put together some Array Tips and Technical Notes that you may find useful.
The Array Tips provide useful tips and quick updates. The Technical Notes are divided by subject category.
Click on the topics below to download pdf files 
If you have any further questions, please contact our Technical Support Specialist:
array tips
Microarray version numbers explained
RNA concentration and clean-up method
brief technical notes
Definition of a high quality microarray experiment (scanned array image)
Evaluation of total RNA isolation methods
technical notes
Platform Validation
Validation of the Illumina iScan System for gene expression
Illumina miRNA BeadChip Validation
Array Production
Corning UltraGAPSTM slides replace CMT-GAPSIITM slides for all UHNMAC microarray products
Automation
Microarray Experiments in a High-Throughput Manner
Protocol Optimisation
Advalytix SlideBooster™ hybridisation station improves hybridisation kinetics for oligonucleotide arrays
Purification of labelled-cDNA
Re-washing microarrays to reduce background
Stability of Cy-labelled cDNA stored at -20C
Use of Cot-1 DNA as hybridisation competitor for CpG island microarrays
Experimental Data
Increasing the number of technical replicates can increase the number of significantly differentially expressed genes